The HBV-N model was used for a scenario analysis of changes in nitrogen retention and transport caused by alterations of wetness due to land drainage, lowering of lakes, building of dams and climatic variability in a river basin in south-central Sweden (1885-1994). In general, dams were situated in locations more favourable for retention, compared to the lowered lakes. Rather modest conversions of water bodies only changed nitrogen transport by about 3%. The 180-times-larger increase of (mainly) tile-drained agricultural land had, according to simulations, increased the nitrogen transport by 17%, due to reduced retention. However, compared to human-induced alteration of the landscape N retention, the choice of 10-year periods of climatological data had the overriding effect on the calculated nitrogen transport. Weather-induced variations resulted in a 13% difference in nitrogen retention between various 10-year periods. When the model was driven by climatological data from the driest 10-year period (1905-1914), the estimated average annual load was only half of that obtained with climatological data from the wettest 10-year period (1975-1984).