During the August-September 2003 Autonomous Ocean Sampling Network-II experiment, the Harvard Ocean Prediction System (HOPS) and Error Subspace Statistical Estimation (ESSE) system were utilized in real-time to forecast physical fields and uncertainties, assimilate various ocean measurements (CTD, AUVs, gliders and SST data), provide suggestions for adaptive sampling, and guide dynamical investigations. The qualitative evaluations of the forecasts showed that many of the surface ocean features were predicted, but that their detailed positions and shapes were less accurate. The root-mean-square errors of the real-time forecasts showed that the forecasts had skill out to two days. Mean one-day forecast temperature RMS error was 0.26 degrees C less than persistence RMS error. Mean two-day forecast temperature RMS error was 0.13 degrees C less than persistence RMS error. Mean one- or two-day salinity RMS error was 0.036 PSU less than persistence RMS error. The real-time skill in the surface was found to be greater than the skill at depth. Pattern correlation coefficient comparisons showed, on average, greater skill than the RMS errors. For simulations lasting 10 or more days, uncertainties in the boundaries could lead to errors in the Monterey Bay region. Following the real-time experiment, a reanalysis was performed in which improvements were made in the selection of model parameters and in the open-boundary conditions. The result of the reanalysis was improved long-term stability of the simulations and improved quantitative skill, especially the skill in the main thermocline (RMS simulation error 1 degrees C less than persistence RMS error out to five days). This allowed for an improved description of the ocean features. During the experiment there were two-week to 10-day long upwelling events. Two types of upwelling events were observed: one with plumes extending westward at point Ano Nuevo (AN) and Point Sur (PS); the other with a thinner band of upwelled water parallel to the coast and across Monterey Bay. During strong upwelling events the flows in the upper 10-20 m had scales similar to atmospheric scales. During relaxation, kinetic energy becomes available and leads to the development of mesoscale features. At 100-300 m depths, broad northward flows were observed, sometimes with a coastal branch following topographic features. An anticyclone was often observed in the subsurface fields in the mouth of Monterey Bay. (C) 2008 Elsevier Ltd. All rights reserved.